Measurements Using HPLC
Start Date
1-8-2024 11:30 AM
End Date
1-8-2024 11:45 AM
Location
ALT 211
Abstract
This summer’s research revolved mostly around using reversed phase High Performance Liquid Chromatography (HPLC) for two different projects. Reverse-phased chromatography is especially advantageous for running biological samples, as the most common mobile phase is water. This results in less solvent usage and less hazardous waste. The first project addressed was an ongoing project under Dr. Wetzel measuring the decomposition of creatine into creatinine in the presence of C. elegans. The work this summer entailed preparing replicates to validate the data and method. The data was then processed using R, which required writing code to read raw data, average trial data, create error bars, and making the graphs look publication ready. While still in progress, the second project consists of measuring caffeine levels before and after mycofiltration. Although caffeine is generally considered a relatively easy analyte for HPLC, the samples are challenging. Pigments and other compounds released into the solutions after filtration with mushrooms require a different instrumental method than existing methods for beverage or environmental samples. The method developed this summer is able to quantitate caffeine levels between 100 ppb and 50 ppm.
Measurements Using HPLC
ALT 211
This summer’s research revolved mostly around using reversed phase High Performance Liquid Chromatography (HPLC) for two different projects. Reverse-phased chromatography is especially advantageous for running biological samples, as the most common mobile phase is water. This results in less solvent usage and less hazardous waste. The first project addressed was an ongoing project under Dr. Wetzel measuring the decomposition of creatine into creatinine in the presence of C. elegans. The work this summer entailed preparing replicates to validate the data and method. The data was then processed using R, which required writing code to read raw data, average trial data, create error bars, and making the graphs look publication ready. While still in progress, the second project consists of measuring caffeine levels before and after mycofiltration. Although caffeine is generally considered a relatively easy analyte for HPLC, the samples are challenging. Pigments and other compounds released into the solutions after filtration with mushrooms require a different instrumental method than existing methods for beverage or environmental samples. The method developed this summer is able to quantitate caffeine levels between 100 ppb and 50 ppm.
