Analysis of ACC2 mutations involving development of cancer using bioinformatics and cut6 in fission yeast as a model organism
Start Date
29-4-2022 3:45 PM
Location
Alter Hall Poster Session 2 - 2nd floor
Abstract
ACC2, Acetyl-CoA carboxylase beta, is a central protein that helps guide the direction of lipid metabolism in the cell. Under normal conditions, ACC2 promotes the cell to either synthesize or break down fatty acids for energy storage or use. When mutations occur in the ACC2 gene this could lead to de-regulation of this process. Cancer cells often exhibit altered lipid metabolism and mutations in critical lipid regulators, such as ACC2, could be the cause of this. Using data from the Catalogue of Somatic Mutations in Cancer (COSMIC), it was found that certain types of cancer cells did in fact have significant mutations in their ACC2 genes. This data was analyzed using the Variant Effect Scoring Tool (VEST) to determine the significance of the mutations. Mutations with significant VEST scores involved a single amino acid substitution that could affect the structure or function of the ACC2 protein. To supplement this data, cut6, a functional homolog of ACC2 found in fission yeast, was analyzed in lab. Pictures of the wild type and mutant cut6 fission yeast strains were taken under a phase contrast microscope. These images were quantified using a cell dimension program and overall, the data showed significant differences in cell length, width, and area between the wild type and mutant cells.
Analysis of ACC2 mutations involving development of cancer using bioinformatics and cut6 in fission yeast as a model organism
Alter Hall Poster Session 2 - 2nd floor
ACC2, Acetyl-CoA carboxylase beta, is a central protein that helps guide the direction of lipid metabolism in the cell. Under normal conditions, ACC2 promotes the cell to either synthesize or break down fatty acids for energy storage or use. When mutations occur in the ACC2 gene this could lead to de-regulation of this process. Cancer cells often exhibit altered lipid metabolism and mutations in critical lipid regulators, such as ACC2, could be the cause of this. Using data from the Catalogue of Somatic Mutations in Cancer (COSMIC), it was found that certain types of cancer cells did in fact have significant mutations in their ACC2 genes. This data was analyzed using the Variant Effect Scoring Tool (VEST) to determine the significance of the mutations. Mutations with significant VEST scores involved a single amino acid substitution that could affect the structure or function of the ACC2 protein. To supplement this data, cut6, a functional homolog of ACC2 found in fission yeast, was analyzed in lab. Pictures of the wild type and mutant cut6 fission yeast strains were taken under a phase contrast microscope. These images were quantified using a cell dimension program and overall, the data showed significant differences in cell length, width, and area between the wild type and mutant cells.
