Module 0: Expanding BASIL
Start Date
29-4-2022 3:45 PM
Location
Alter Hall Poster Session 2 - 3rd floor
Abstract
The Biochemistry Authentic Scientific Inquiry Lab (BASIL) curriculum consists of a series of 11 in silico and wet lab modules used to enhance the undergraduate Biochemistry lab experience. These modules can be used to evaluate the function of proteins with known structures but unassigned functions. Currently, the modules are focused on the studying of the serine proteases class of enzyme. The purpose of this research is to develop a Module 0 to find new protein candidates from different protein classes in order to expand BASIL. ASSAM (Amino Acid Search for Substructures and Motifs) was used to find enzymes in the Protein Data Bank with specific arrangements of amino acids. A file parser was developed to sort the ASSAM output to identify proteins containing these motifs but with unclassified functions. The proteins of interest could then be taken through the remaining BASIL modules. The use of ASSAM and the file parser allowed for an efficient expansion of BASIL into new candidates.
Module 0: Expanding BASIL
Alter Hall Poster Session 2 - 3rd floor
The Biochemistry Authentic Scientific Inquiry Lab (BASIL) curriculum consists of a series of 11 in silico and wet lab modules used to enhance the undergraduate Biochemistry lab experience. These modules can be used to evaluate the function of proteins with known structures but unassigned functions. Currently, the modules are focused on the studying of the serine proteases class of enzyme. The purpose of this research is to develop a Module 0 to find new protein candidates from different protein classes in order to expand BASIL. ASSAM (Amino Acid Search for Substructures and Motifs) was used to find enzymes in the Protein Data Bank with specific arrangements of amino acids. A file parser was developed to sort the ASSAM output to identify proteins containing these motifs but with unclassified functions. The proteins of interest could then be taken through the remaining BASIL modules. The use of ASSAM and the file parser allowed for an efficient expansion of BASIL into new candidates.
